Preparation of potato flour slurry
Slurries of various concentrations (10, 15, 20, 25 and 30% w/v) of
potato flour starch was prepared in water and treated with liquefying
enzyme (0.15% v/w) at 104 to 105°C for 60 min in an autoclave.
The slurry prepared by mixing 25 g flour in 100 ml water (1:4) being
homogenous, loose, easy to handle was used f or further experiments. Liquefaction of potato flour (100 ml slurry) was carried out at
104 to 105°C in an autoclave using varying concentration of alpha
amylase (0.05 to 0.20% v/w) for different time intervals (10 to 240
min). The progress of liquefaction was monitored by employing
starch-iodine (1 g of iodine and 2 g KI in 100 ml water) reaction.
Saccharification of liquefied starch was carried out at 60°C for
different time intervals using varying concentration (0.05 to 0.45%
v/w) of amyloglucosidase. The reaction was monitored by the yield
of total reducing sugars estimated by dinitrosalicylic acid method
(Miller, 1959)
Preparation of potato flour slurry
Slurries of various concentrations (10, 15, 20, 25 and 30% w/v) of
potato flour starch was prepared in water and treated with liquefying
enzyme (0.15% v/w) at 104 to 105°C for 60 min in an autoclave.
The slurry prepared by mixing 25 g flour in 100 ml water (1:4) being
homogenous, loose, easy to handle was used f or further experiments. Liquefaction of potato flour (100 ml slurry) was carried out at
104 to 105°C in an autoclave using varying concentration of alpha
amylase (0.05 to 0.20% v/w) for different time intervals (10 to 240
min). The progress of liquefaction was monitored by employing
starch-iodine (1 g of iodine and 2 g KI in 100 ml water) reaction.
Saccharification of liquefied starch was carried out at 60°C for
different time intervals using varying concentration (0.05 to 0.45%
v/w) of amyloglucosidase. The reaction was monitored by the yield
of total reducing sugars estimated by dinitrosalicylic acid method
(Miller, 1959)
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