acuole fragmentation in Δatg18 cells is retarded. (A) Δatg18 (BJ3505) cells were stained with FM4-64 (red) and imaged at the indicated times after salt addition. Arrows mark intravacuolar spherical structures. (B) Quantification of the fragmentation of Δatg18 vacuoles. Compare with the graph for wild-type cells in Figure 2C. (C) Localization of Fab1p in Δatg18 cells. Wild-type and isogenic Δatg18 cells carrying Fab1-GFP were grown logarithmically in YPD. Fab1-GFP localization was analyzed by confocal fluorescence microscopy