Therefore, in order to counteract the
surface forces on the drop, it was necessary to apply the pressure
that was 6mbar for 60 s from outlet to inlet after every extraction
for 120 s. After completion of the extraction, about 0.13 nL of the
IL drop enriched with the sample was pressed into the capillary
under 30 mbar for 5 s. The IL drop remaining after the injection fell
off automatically. Finally, the separation process was running under
20 kV with 20mmolL−1 borate (pH 10.0) including 10% methanol
(v/v) as the run buffer.