Method I conditions (C10 alkane and milk fat FAMEs): inj. vol.:0.2 L (C10) and 0.5 L (FAMEs); split ratio: 200:1; GC1/GC2 temp.program: 50–280 ◦C at 5 ◦C/min; initial inlet pressure: 45.5 kPa;initial auxiliary pressure: 3.9 kPa; in both dimensions the linearvelocity was maintained constant. Stainless steel accumulationloops measuring 20 cm and 46 cm (×0.71 mm OD × 0.51 mm ID) were used (SGE). Modulation period: 3.1 s (accumulation period 2.7 s/injection period 0.4 s).