The formation and stability of Ag nanoparticles were followed by
UV–vis spectrophotometry (Cary, UV-50, Japan). The absorption spectrum
of reaction solutions as a function of reaction time and different
biomaterials and AgNO3 dosage at the same time were recorded at
wavelengths ranging from 300 to 600 nm. Distilled water was used as
a blank solution. Stability of bio reduced silver nanoparticles was analyzed
using UV–Vis absorption spectra. For this 4-fold diluted solution
was prepared.