Although several studies, which evaluate the antimicrobial activity of natural extracts, can be found in the literature, those obtained with the use of CO2 as the solvent have not been extensively investigated. In this context, this manuscript represents an extension of our previous work [29] on the extraction and evaluation of ginger extraction. The main objective of this work was to obtain and compare the effects of the extraction method on the chemical composition and antibacterial activity of ginger extracts obtained from supercritical CO2 extraction and hydrodistillation processes.