Materials and methods
Construction of codon-optimized Caenorhabditis elegans (C. elegans) fat1 gene expression vector
The mfat1 gene was cloned from the coding region of C. elegans fat1 gene (GenBank accession number NM_001028389) and codon-optimized according to the mammalian codon usage bias in order to be expressed in mammalian cells (Supplementary
Table 1). The mfat1 gene expression vector-pST200 was consisted of a muscle creatine kinase enhancer and a cytomegalovirus enhancer with a chicken bactin promoter. A PGK/neo expression cassette flanked by the loxP sites was linked to the backbone as the selection marker (Fig. 1).