Two prototypes of cryosurgery devices consisting of 5- and 6-stage TEC modules were experimentally investigated under variations of electrical voltages, circulating thermostatic bath (CTB) temperatures, and heat exchanger configurations in order to obtain the modules’ characterizations. The multi-stage TEC modules prototypes have shown good results as a cooling source for the cryosurgery cooling system and are ready for clinical trials. The following conclusions are also presented: