Inhibition of protein synthesis
Inhibition ofproteinsynthesiswasperformedinATCC
700392Helicobacter pylori strain by 35S methionineincorporation
assay byfollowingthemethodof Patrzykat etal.(2002). Gut-
Gards was testedatconcentrationsof4and8 mg/ml whilethe
positive control,tetracyclinehydrochloride,wastestedat0.03 mg/
ml. ATCC700392Helicobacter pylori strain wasinoculatedin
Brucella brothsupplementedwith7%horseserumandincubated
for 72h.Thenthecellswereharvestedbycentrifugationandre-
suspended inwarmsyntheticM9mediumplusBrucellabrothand
incubated with 35S methioninefor10min.Afterincubation,the
cells werepelletedandwashedtwicewithM9medium.There-
after, itwasre-suspendedinM9mediumplusBrucellabroth
and incubatedwithandwithouttestsamplesfor48hat35 1C
in anincubatorundermicroaerophiliccondition.Controls
(Organismþ35S Methionine)withoutsamplewereincludedin
the study.