One gram of each soil sample was shaken in 100 mL of phosphate buffered saline.
Then, serial dilutions of each suspension were plated on King’s B agar (KB) medium: 20 g L-1 bactopeptone, 1 g L-1 K2HPO4, 1.5 g L-1 MgSO4 7 H2O, 10 mL L-1 glycerol, 15 g L-1 agar. After incubation at 28 C for 24 h, representative colonies were selected for further studies.
One gram of each soil sample was shaken in 100 mL of phosphate buffered saline.Then, serial dilutions of each suspension were plated on King’s B agar (KB) medium: 20 g L-1 bactopeptone, 1 g L-1 K2HPO4, 1.5 g L-1 MgSO4 7 H2O, 10 mL L-1 glycerol, 15 g L-1 agar. After incubation at 28 C for 24 h, representative colonies were selected for further studies.
การแปล กรุณารอสักครู่..
