However, BSPO-F2 primer was replaced by the forward internal primer designed in this study (5'AGAAGAGCGGAATTCCAC3'), which shows a C as the last 3' nucleotide, representing the only nucleotide different from B. acidicola in this fragment, according to the sequences deposited in GenBank (ID: EU231617.1; ID: AF329476.1; ID: 49080.1).