Test
The production of ammonium gas leads to the
change in the pale yellow colour of the urease medium
incorporated with about 0.3ml of urea bright pink
colour.
Heavy inoculum was obtained from the test
isolates and inoculated on a slope of the medium in
universal bottle and was incubated for 24hrs at 370C. A
pink-red colour indicates a positive test while a negative
result retains the colour of the urease medium [4].