The Ames and the Zimmermann tests are being able to demonstrate nuclear DNA effects such as point mutations, gene reversion and gene conversion to assess the mutation induction after exposure to drinking water samples on bacterial and yeast DNA. In vitro alkaline single cell gel electrophoresis or comet assay on HepG2 cells involves detection of cell DNA fragments under alkaline conditions, which during electrophoresis, migrate from the nuclear core, resulting in “a comet” formation. Comet assay is a method for DNA alkali- labile sites and strand breaks detection in individual cells and is one of the major tools in environmental pollution biomonitoring, both in vivo and in vitro.13,14,15
The aim of the present study was to compare