2.8. Total phenolics content (TP) from carrot juice and yoghurt
2.8.1. From carrot juice
The TP content was determined by the Folin-Ciocalteu method
after extraction from carrot juice with 30 mL of solvent (80%
aqueous ethanol, containing 1% conc. HCl) in a conical flask,
agitating in an orbital shaker (200 rpm, at 50
C, for 2 h),filtering
(Whatman No. 4) as described in Lima et al. (2005). The filtered
extract was used for the determination of TP content and ferric
reducing power.
The extract (100mL) was mixed with 750mL of Folin-Ciocalteu
reagent, allowed to stand at room temperature for 5 min and
mixed gently with 750mL of 6% (w/v) sodium carbonate. A blank
was made by mixing distilled water and reagents. After allowing to