A total no. of 50 urine samples was processed. Out of these samples, 15 isolates were confirmed as E.coli by Gram’s staining and biochemical tests. Rest of these samples showed growth of other microorganism like as Klebsiella species, Staphylococcus species, Bacillus species etc. Out of 15 E.coli the isolation rate in urine samples was high in females in comparison to males.