2.4. Effect of dilution rate on the quality of fermented MLBBR
beverage
To determine the effect of quantity of BRJ on the quality of fermented
MLBBR beverage, product was prepared using ground
Moringa leaves paste and BRJ in 1:1, 1:2; 1:3 and 1:4 ratios and
sterilized. These were inoculated with L. plantarum and E. hirae at
1% level (with 107 cfu/ml in the inoculum) and allowed to ferment
for 48 h at 37 C.
2.5. Determination of proteolytic activity of the lactic cultures
The Moringa leaves based juicewas fermented with L. plantarum
and E. hirae, individually, and the proteolytic activity of these cultures
in fermented Moringa leaves based juice was determined
using nutrient agar supplemented with 2% skim milk powder
(SMP). The sterile SMP containing nutrient agar was poured in
sterile Petri plates and well were made with sterile SS borer (8 mm
dia.) and filled with 100 ml of particle free clear juice of fermented
Moringa leaves. The plates were kept at 10 C for 2 h and later
shifted to an incubator maintained at 37 C for 24 h. After