Probably related to the surge of ESBL producers, a survey of carbapenem use in Thailand during 2010–2013 showed a significant increase from approximately 2.1 to 3.1 million vials per year. However, very little is known about the molecular epidemiology and genetic characteristics of CRE in Thailand. Here we investigated the prevalence and resistance characteristics of CRE at the largest university hospital in Thailand with an emphasis on the characterization and molecular typing of CRKP.