BATH test was performed according to an established protocol
(Collado et al., 2007). Bacterial cells were washed with PBS buffer
and resuspended in PBS. Initial O. D A600 adjusted to 0.2 to 0.3 to
optimized bacterial cells 107 cells/ml. Equal proportion of xylene
was added to bacterial cells and vortexed well about 5 min and
incubated for 1 h at room temperature. Two distinct phases can
then be seen, aqueous phase was removed and reading was noted.
BATH% was calculated as follows: