Samples were preheated for 5 min at 95 C and then amplified using 30 cycles of 1 min at 95 C, 1 min at 65 C, and 1 min at 72 C. The PCR products were purified using a gel and PCR puri- fication system kit
samples สำหรับ preheated โดย min at 95 c ( then ทั้งปี 30 cycles ของ 1 min at 95 c กลัว min at 65 c , ( 1 min at 72 c. products the pcr สำหรับ purified gel ( pcr puri จะ kit system fication