Here, we report a
robust tissue culture system for sorghum, which has minimized these major impediments. To optimize media,
different concentrations of various plant growth regulators, such as 2,4-dichlorophenoxyacetic acid (2,4-D),
N6
-benzyladenine (BA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthaleneacetic acid
(NAA) were evaluated