Plant Material
Agave potatorum Zucc. plants were obtained from a micro-propagation (tissue culture) laboratory. Tissue culture was done with the mineral salt formulation of Murashige and Skoog (1962), with 1.5 mg/L of benzil-adenine (BA), 0.4 mg/L of thiamine, 100 mg/L of myo-inositol, and 30 g of sucrose as supplement. The plant acclimatization period was 60 days using perlite as the substrate.