Flowering is a complex process regulated by genetic and environmental factors. In order to study this morpho-physiological event in the plant cycle, it is important to have a reliable bioassay system. In this respect, valuable information of the flowering process has been obtained in previous studies using in vitro plants as reported by Zhang (2007), or using homogeneous African violet plants (Saintpaulia ionantha Wendl.) obtained via micropropagation (Martin-Mex et al., 2005). Since micropropagated plants are sensitive to external application of plant regulators, the present research used gloxinia (Sinningia speciosa Benth.) vitroplants to evaluate if the flowering is affected by SA (Larqué-Saavedra et al., 2007)