For osmotic pretreatment, cumin seeds were placed on a
PEG6000 solution at
−0.8 and
−1.2 Mpa of polyethylene glycol
(PEG6000) at 15 ◦C for 48 h under dark conditions. The concentration
of the PEG solution was calculated according to Michel (1983). After
priming, the seeds were rinsed with distilled water three times and
dried for 48 h at 25 ◦C to the original moisture content (∼12–13%)
as the unprimed seeds and immediately used for germination tests.