The present authors have previously reported the partialpurification of cell wall glucan by using the NaClO oxidationmethod (21). Oxidized A. fumigatus cells were preparedfrom the β-glucan (laminarin)-added and controlcultures, and their structure analyzed by 13C-NMR spectroscopyand enzymolysis using zymolyase, endo β-1,3-glucanase. The amount of zymolyase-susceptible componentwas examined (Table 2). In the sucrose and glucoseaddedcultures, it was lower than the detection limit(<5%). In the β-glucan-added culture, this value increasedto 13.5%.
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