4.2. Headspace volatile analysis of blossoms
Blossoms (3 g) were placed into a 42 mL glass vial capped with a
Teflon-faced silicone septa. Samples were equilibrated for 20 min
at room temperature (RT) prior to analysis. A SPME device from
Supelco (Bellefonte, PA) with 1 cm fiber coated with 75 lm CAR/
PDMS was used to collect volatiles. In addition, to initial conditioning,
the fiber was conditioned at 200 C for 5 min prior to each
blossom sampling. The SPME fiber was exposed in equilibrated
blossom headspace for 30 min at RT to collect volatiles.