Total antioxidant capacity using phosphomolybdate method: 0.3 mL of wine sample was mixed with 3 mL reagent solution (6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate. The reaction mixture was incubated at 95°C for 90 min. The mixture was cooled to room temperature and the absorbance of the solution was measured at 695 nm against blank. Based on the reading the percentage scavenging activity was calculated (Prieto et al. 1999).