Although there have been many investigations of the beneficial effects of both exercise and amino acids (AAs),
little is known about their combined effects on the single-dose ingestion of AAs for lipid metabolism during
exercise. We hypothesize that taking a specific combination of AAs implicated in glucagon secretion during
exercise may increase fat metabolism. We recently developed a new mixture, d–AA mixture (D-mix), that
contains arginine, alanine, and phenylalanine to investigate fat oxidation. In a double-blind, placebo-controlled
crossover study, 10 healthy male volunteers were randomized to ingest either D-mix (3 g/dose) or placebo.
Subjects in each condition subsequently performed a physical task that included workload trials on a cycle
ergometer at 50% of maximal oxygen consumption for 1 hr. After oral intake of D-mix, maximum serum
concentrations of glycerol (9.32 ± 6.29 mg/L and 5.22 ± 2.22 mg/L, respectively; p = .028), free fatty acid level
(0.77 ± 0.26 mEq/L and 0.63 ± 0.28 mEq/L, respectively; p = .022), and acetoacetic acid levels (37.9 ± 17.7
μmol/L and 30.3 ± 13.9 μmol/L, respectively; p = .040) were significantly higher than in the placebo groups.
The area under the curve for glucagon during recovery was numerically higher than placebo (6.61 ± 1.33
μg/L • min and 6.06 ± 1.23 μg/L • min, respectively; p = .099). These results suggest that preexercise ingestion
of D-mix may stimulate fat metabolism. Combined with exercise, the administration of AA mixtures could
prove to be a useful nutritional strategy to maximize fat metabolism.
Although there have been many investigations of the beneficial effects of both exercise and amino acids (AAs),little is known about their combined effects on the single-dose ingestion of AAs for lipid metabolism duringexercise. We hypothesize that taking a specific combination of AAs implicated in glucagon secretion duringexercise may increase fat metabolism. We recently developed a new mixture, d–AA mixture (D-mix), thatcontains arginine, alanine, and phenylalanine to investigate fat oxidation. In a double-blind, placebo-controlledcrossover study, 10 healthy male volunteers were randomized to ingest either D-mix (3 g/dose) or placebo.Subjects in each condition subsequently performed a physical task that included workload trials on a cycleergometer at 50% of maximal oxygen consumption for 1 hr. After oral intake of D-mix, maximum serumconcentrations of glycerol (9.32 ± 6.29 mg/L and 5.22 ± 2.22 mg/L, respectively; p = .028), free fatty acid level(0.77 ± 0.26 mEq/L and 0.63 ± 0.28 mEq/L, respectively; p = .022), and acetoacetic acid levels (37.9 ± 17.7μmol/L and 30.3 ± 13.9 μmol/L, respectively; p = .040) were significantly higher than in the placebo groups.The area under the curve for glucagon during recovery was numerically higher than placebo (6.61 ± 1.33μg/L • min and 6.06 ± 1.23 μg/L • min, respectively; p = .099). These results suggest that preexercise ingestionof D-mix may stimulate fat metabolism. Combined with exercise, the administration of AA mixtures couldprove to be a useful nutritional strategy to maximize fat metabolism.
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