2.5. In vitro proteins release
BSA was used as model protein to examine the smart
release behavior of the superabsorbent hydrogels [35].
Drug-loading was carried out by reswelling the dried samples
in BSA solution for 3 days. After the reswelling equilibrium
was reached, the drug-loaded hydrogels were
immersed in a phosphate buffer solution (PBS) solution
(pH 7.4) at 37 C to determine the release of the BSA. The
BSA amount released in the solution was detected by ultraviolet–
visible spectrophotometry (Shimadzu UV-160A).
With each sampling, the solution was changed with fresh
medium, while maintaining a constant total volume. All