RAW 264.7 (murine macrophage) and HT-29 (human colon adenocarcinoma) cell lines were purchased from the Korean Cell Line Bank (KCLB; Seoul, Korea). Each medium of RAW 264.7 and HT-29 cell lines were DMEM (Invitrogen, Grand Island, NY, USA) and RPMI 1640 (Invitrogen). Both medium were containing 10% FBS (Invitrogen) and 1% streptomycin-penicillin (Invitrogen), and incubated under 5% CO2 at 37 °C.