2.2. Chemicals and reagents
HPLC grade sodium hydroxide and sodium acetate
trihydrate were obtained from Fisher Scientific.Millipore water (18 M) was used to prepare eluents
and samples. Standard sugars and sugar alcohols were
analytical grade. Sodium azide was from Sigma. 1-Kes-
tose, 1,1 nystose and 1,1,1 fructofuranosylnystose were
generously donated by Dr. Takahisa Tokunage of Meiji
Seika Kaisha, Ltd. Theanderose (6-a-d-glucosylsucrose)
was purchased from Wako chemicals and kindly pur-
ified by Dr. Greg Cote of USDA-ARS. Absolute etha-
nol was from Aaper.