The purpose of this paper was to examine the flavonoids extracts from bamboo shoot shell.Based on the single factor analysis,a 4-factor,3-level Box-Behnken experimental design was taken to evaluate the effects of the four factors,including ethanol concentration,ratio of material to liquid,extraction time,extraction temperature.Meanwhile the interactions of each factor on the extraction yield were also investigated with response surface analysis by Design Expert soft.The results showed that the optimal extracting conditions were:ethanol concentration was 60%,ratio of material to liquid was 1:25,extraction time was 30min,extraction temperature was 60℃,and under these conditions the extraction yield was 0.80%.