2.2. Media preparation
Preparation of Conway medium involves the preparation of stock solutions which consisted of the macronutrients, trace metal solutions and vitamins. For the preparation of stock solutions, the chemical compositions were diluted in distilled water. Chemical composition of Conway medium was shown in Table 1. One milliliter of macronutrient, 0.5 mL of trace metal, and 0.1 mL of vitamins were added to 1000 mL of filtered and sterilized seawater at 30 ppt salinity prior to the inoculation and maintenance of pure microalgae seed culture.