40 s, and was completed by a final extension (72 °C for 8 min). The reaction products were separated electrophoretically through an 8% non-denaturing polyacrylamide gel, and visualized by silver staining.
40 s, and was completed by a final extension (72 °Cfor 8 min). The reaction products were separatedelectrophoretically through an 8% non-denaturingpolyacrylamide gel, and visualized by silver staining.