2.3.5 Effect of botanicals on anthracnose fungi spore
germination
On the effect of plant extracts on spore germination, slide
technique method of [1] was used. Each plant extract was added
to dried clean slides as films and then 0.1 millileter of spore
suspension of fungal pathogen was sprayed over the films with
hand sprayer while a control was prepared as a film of distilled
water. Three slides were, used as replicates for each plant
extract. Each slide was, placed on glass rod in Petri dish under
moistened conditions and incubated for 24 hours at 25oC. Using 4
microscopic fields (x = 10 x 40) for each replicate, spore
germination (SG) percentages were calculated using the following
formula;