The present study was carried out
to optimize in vitro shoot multiplication protocol for two selected sugarcane varieties (B41-227 and N14) widely grown in Ethiopian
sugar estates to complement with the conventional propagation method. In the study, initiated aseptic shoot tip cultures of the two
sugarcane varieties were treated with four concentrations (1.5, 2, 2.5 and 3 mgL-1) of 6- benzylaminopurine (BAP) and Indole-3-acetic
acid (IAA) (0.25, 0.5, 0.75 and 1 mgL- 1) while Plant growth regulator free medium was used as free check (control)