4. Conclusion
Since the microorganism plays a significant role in final fermented
products, our findings have contributed a further step in
improvement of traditional fermented tea. The new designed
symbiosis 4Y6A between the isolated yeast (D. bruxellensis KN89)
and AAB (G. intermedius KN89) which showed the highest glucuronic
acid production up to 175.8 ± 7(mg L1) in 7-day fermentation
is ready for further applications in pilot scale of production.
Moreover, identification of specific growth rate of G. intermedius
KN89 and the velocity of GlcUA formation have provide a critical
time point for intervention of fermentative technology. After 54 h
of the process, G. intermedius had reached its stationary phase and
increased the GlcUA production velocity. It was the most suitable
time to modify the process by supplying more nutrition, growth
factors, or switching from batch fermentation to continue
fermentation in order to prolong the high potential stage and
obtain large amount of GlcUA. However, optimization of fermentative
conditions for this newdesigned symbiosis is required before
industrial application. These preliminary findings are essential for a
high quantity and quality of GlcUA-rich kombucha production.