Amniotic membrane
transplant
Preserved human amniotic membrane is introduced into the surgical
field after gently irrigating the membrane with sterile balanced salt solution, and it is removed from its carrier sheet using smooth tying forceps
(Figure 5).
The membrane is oriented on the
carrier sheet with its stromal side in
contact with the carrier sheet. When
the membrane is about 60% released
from the carrier sheet, the carrier sheet
is turned upside down and the amniotic membrane is pulled from the carrier sheet. This ensures the membrane
lands on the ocular surface with the
stromal side down (Figure 5).
The membrane is spread evenly,
so that it covers the bare scleral region
and the peripheral cornea past the surgical limbus (Figure 5). The membrane
may be anchored using two 10-0 Vicryl
sutures on the conjunctival region.