Figure 3. Chromatograms showing the specificity of the high-performance liquid chromatography (HPLC) method for calcium propionate. Chromatograms of calcium propionate (A) in a bread sample, (B) standard calcium propionate spiked sample, and (C) standard calcium propionate. Chromatographic conditions: the column was a Symmetry C18 HPLC column (4.6 mm × 150 mm., i.d.,5 μm); the flow rate was 1.0 mL/min; the mobile phase was methanol and 0.05% phosphoric acid (10:90, v/v); the injection volume was 20 μL; UV detection occurred at 210 nm; and the run time was 9 minutes.