The FRAP assay was performed according to the
procedure described by Benzie and Szeto [13].
The FRAP reagent was prepared from 300 mM
sodium acetate buffer (pH 3.6), 10 mM TPTZ
dissolved by 40 mM HCl and 20 mM ferric
chloride in the volume ratio of 10:1:1. The
different concentrations (400-800 µg/ml) of
sample was dissolved using methanol as a
solvent. 25 µl of each concentration of the
sample was added to 175 µl of FRAP reagent.
Blank experiment contained 25 µl of sample and
175 µl of sodium acetate buffer. The absorbance
of the mixtures was measured at 593 nm using
UV– spectrophotometer (DKSH). Ascorbic acid in
methanol was used as a positive control and the