The FRAP assay was performed according to the 
procedure described by Benzie and Szeto [13]. 
The FRAP reagent was prepared from 300 mM 
sodium acetate buffer (pH 3.6), 10 mM TPTZ 
dissolved by 40 mM HCl and 20 mM ferric 
chloride in the volume ratio of 10:1:1. The 
different concentrations (400-800 µg/ml) of 
sample was dissolved using methanol as a 
solvent. 25 µl of each concentration of the 
sample was added to 175 µl of FRAP reagent. 
Blank experiment contained 25 µl of sample and 
175 µl of sodium acetate buffer. The absorbance 
of the mixtures was measured at 593 nm using 
UV– spectrophotometer (DKSH). Ascorbic acid in 
methanol was used as a positive control and the