RAW 264.7 macrophages (ATCC number: TIB-71) were cultured
in Dulbecco’s Modified Eagle Medium supplemented with
10% (v/v) non-inactivated fetal bovine serum, 100 U/mL penicillin
and 100 g/mL streptomycin. The cells were cultured at
37 °C in a humidified atmosphere of 95% air and 5% CO2.