Sequence alignments were implemented with SeqScape v.2.1.1 software (Applied Biosystems). Measuring Genetic
differentiation was based on Kimura two parameter (K2P) distance, a suitable metric model when genetic distances are low
(Nei and Kumar, 2000). The unrooted neighbor-joining (NJ) tree was established in MEGA 3 (Kumar et al., 2004). The
correlation between geographical distances and genetic distances was analyzed by the Mantel test in R 2.9.2 software (R
Development Core Team, 2008).