2.6. Seed germination assays
To determine the seed germination rate, 120 seeds of T2 generation of different transgenic lines and WT were sterilized and spreadon half strength MS medium without sugar (pH 5.8). Seeds wereplaced in a growth chamber with 12/12 light/dark cycle at 28◦C. Germination is defined as the emergence of the radicles through theseed coat. Every experiment was conducted with three replicates