In this study, autolysis was induced by incubating cell suspensions of spent brewer’s yeast
at elevated temperatures of 45, 50, 55 and 60 8C with a reaction time ranging from 8 to 72 h.
Contents and yields of solid, a-amino nitrogen, protein and carbohydrate were determined.
It can be said that optimum temperature and time for the production of yeast extract was
50 8C for 24 h on the basis of a-amino nitrogen and protein contents. Sensory analysis was
also done by yeast extract powder and control and samples with the addition of 0.5 and 1.0%
yeast extract had the highest overall acceptance.
#2007 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.