2.3. Haemolymph measurements
Glucose levels were measured by the glucose–oxidase
method, using a Bioclin Kit (glucose GOD-CLIN). Results
are expressed in mmol/L. Proteins were measured following the
method described by Lowry et al. (1951), using bovine albumin
(Sigma-Aldrich Chemical Co., St. Louis, MO, USA) as the
reference substance. Results are expressed in mg/mL. Total
lipids were measured by the sulfophosphovanillin method
(Frings and Dunn, 1970), with the results expressed in mg/L.
Total cholesterol was measured using a Labtest kit (Total
Cholesterol Liquiform), with the results expressed in mg/mL.
Triglycerides were measured using a Biodiagnostic Kit (GPO
Trinder), and are expressed in mg/L.
2.3. Haemolymph measurementsGlucose levels were measured by the glucose–oxidasemethod, using a Bioclin Kit (glucose GOD-CLIN). Resultsare expressed in mmol/L. Proteins were measured following themethod described by Lowry et al. (1951), using bovine albumin(Sigma-Aldrich Chemical Co., St. Louis, MO, USA) as thereference substance. Results are expressed in mg/mL. Totallipids were measured by the sulfophosphovanillin method(Frings and Dunn, 1970), with the results expressed in mg/L.Total cholesterol was measured using a Labtest kit (TotalCholesterol Liquiform), with the results expressed in mg/mL.Triglycerides were measured using a Biodiagnostic Kit (GPOTrinder), and are expressed in mg/L.
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