Gas chromatographyemass spectrometry was used to identify
and quantify the various amino acids in fresh seeds, seeds coldstratified
for 4 and 8 weeks and radicle-emerged seeds; a minimumfive
replications of each samplewas used. The methods for this
analysis followed those of the Metabolomics Fiehn Lab, UC Davis,
http://fiehnlab.ucdavis.edu/publications/Arabidopsis Protocols.