Lastly, we assessed the cooked pork LFD using cooked pork spiked
into a cooked beef background extracted using the standard 0.9% saline
protocol used for the raw meat extraction method (Method A). Using
this device, a convincing positive T1 line value (exceeding the 60 unit
threshold) was reproducibly achieved for 1.0% cooked pork contamination
(Table 4). Thus, the cooked pork LFD, based on antibodies raised
against the thermostable meat proteins, could be used to detect cooked
pork residues without the need of using special extraction conditions.