This started the eclosion process. The emerged larvae were maintained in Petri dishes with dechlorinated tap water. Larvae were fed with a diet of yeast and dog biscuits in the ratio of 3:1.The first in star larvae developed into pupae in about 7-14 days through four stages. The pupae were separated by using a glass dropper into glass Petri dishes and were kept in mosquito net cages for emergence.