The reducing power was measured as described by Zhao et al. (2008). The sample (500 mg, dry weight basis) was weighed in polypropylene tubes and 0.5 mL of 80% methanol was added and shaken on shaker for 2 h. After that, the tubes were centrifuged at 3000g for 10 min and supernatant (extract) was collected. The extract (1 mL) was mixed with phosphate buffer (2.5 mL) and 2.5 mL potassium ferricyanide was added followed by incubation at 50 C. Trichloroacetic acid solution (10 g/100 mL) was added to mixture, which was then centrifuged at 10,000g for 10 min. The upper layer of solution (2.5 mL) was mixed with 2.5 mL deionized water and 0.5 mL ferric chloride. The absorbance of the mixture was measured at 700 nm. A standard curve was prepared using various concentration of ascorbic acid and results were reported as ascorbic acid equivalents (AAE)/g of flour.