Cell cultures were subjected to embryo regeneration conditions by subculturing approximately 200 mg callus every 4 weeks twice on ‘R’ regeneration medium containing 17.76 lM 6-BA and twice on ‘M’ maturation medium containing 1.35 lM 6-BA until plantlets developed. Plantlets exhibiting one leaf pair were transferred to the same maturation medium devoid of plant growth regulators and enriched with 1 g/L active charcoal until acclimatization.