2.2.8. Polarization microscopy
The prepared samples were screened by Carl Zeiss ApoTome Imager Z1 microscope (Zeiss, Göttingen, Germany) integrated with digital AxioCam ICc1 and AxioVision 4.6 computer software. A pin-tip amount of each sample was taken from three separate sites within the sample and smeared onto a glass microscope slide covered with cover slip and pressed to make the film as thin as possible. Photographs at a magnification of 400× (bar 20 μm) were captured under cross-polarization in the bright field using a wavelength (λ) plate to detect birefringence. The mean droplet diameter was determined by analyzing droplets (N = 100) in the micrograph area (1380 × 1030 pixels) with the software AxioVision 4.6.